Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: Fibronectin , REAL555 , 50 , 130-122-864 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: Cell reports

Article Title: Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates

doi: 10.1016/j.celrep.2020.107599

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 1 × 10 6 cells were plated over 10 cm culture plates (Falcon, 35–3003), previously coated with poly-L-ornithine (PLO) (Sigma, P3655) and fibronectin (FN) (R&D Systems, 1030FN), and incubated at 37°C, 5% O 2 and 5% CO 2 for 5 days in DMEM/F12 medium (Mediatech 16–405-CV) plus N2 supplement, containing 25 μg/ml bovine insulin (Sigma, I6634), 100 μg/mL apotransferrin (Sigma, T2036), 20 nM progesterone (Sigma, P8783), 100 mM putrescine (Sigma, P5780), 30 nM sodium selenite (Sigma, S5261), penicillin/streptomycin (Life Technology, 15140–122).

Techniques: Virus, Plasmid Preparation, Recombinant, Transfection, Antibody Labeling, In Vitro, Microarray, Gene Expression, Derivative Assay, Software

Journal: Molecules

Article Title: Vincamine Ameliorates Epithelial-Mesenchymal Transition in Bleomycin-Induced Pulmonary Fibrosis in Rats; Targeting TGF-β/MAPK/Snai1 Pathway

doi: 10.3390/molecules28124665

Figure Lengend Snippet: Effect of vincamine on fibronectin ( A ), N-cadherin ( B ), and collagen ( C ) levels in lung tissues. Bars represent mean ± SD. Significant difference was analyzed by one-way ANOVA test followed by post hoc Dunnett test, where * p < 0.001, compared to sham group, and # p < 0.001, compared to BLM-induced group.

Article Snippet: In lung tissue homogenates, levels of fibronectin, N-cadherin, and collagen were also assessed, according to the manufacturer’s instructions, utilizing rat fibronectin ELISA kit (#MBS761397, MyBioSource, CA, USA), N-cadherin ELISA kit (#KOA0665, Rockland immunochemical Inc., Royersford, PA, USA), and collagen type I ELISA kit (#MBS262647, MyBioSource, CA, USA), respectively.

Techniques:

Journal: bioRxiv

Article Title: Effect of therapeutic ultrasound on the mechanical and biological properties of fibroblasts

doi: 10.1101/2021.11.22.469508

Figure Lengend Snippet: Protein concentration in cell culture supernatants of ligament fibroblasts. Values were measured on the 6 th day and 10 th day after stimulation period for type I collagen, type III collagen, and fibronectin.

Article Snippet: We used rat collagen type I (E-EL-R0233), rat collagen type III (E-EL-R0235), and rat fibronectin (E-EL-R0578) Elabscience® ELISA kits.

Techniques: Protein Concentration, Cell Culture

Journal: Chinese Medical Journal

Article Title: Exosomal miR-485-3p derived from pancreatic ductal epithelial cells inhibits pancreatic cancer metastasis through targeting PAK1

doi: 10.1097/CM9.0000000000002154

Figure Lengend Snippet: Suppressive effects of Exo hTERT-HPNE on PC cells and PSCs through delivering mature miR-485-3p into the corresponding cells. (A) Transwell migration and invasion assays of BxPC-3 and PANC-1 cells treated with or without Exo hTERT-HPNE (bar: 100 μm). Cells were imaged at 200 × magnification in five random fields and counted using ImageJ software. (B) Transwell migration assay of PSCs treated with or without Exo hTERT-HPNE (bar: 100 μm). (C) FN1, COLIA1, and FAP levels in cell supernatant of PSCs treated with or without Exo hTERT-HPNE assessed by ELISA. (D) Detection of miR-485-3p in exosomes of hTERT-HPNE, PSCs, and PC cells, as well as in the corresponding parental cells using RT-PCR. MiR-485-3p was not detected in Exo PSC , Exo BxPC-3 , and Exo MIA PaCa-2 . U6 served as endogenous reference. (E) Mature miR-485-3p and its precursors (pre-miR-485-3p, pri-miR-485-3p) of PSCs and PC cells were detected by RT-PCR. U6 and GAPDH, respectively, served as endogenous reference of miRNA and mRNA. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; miRNAs: microRNAs; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells; RT-PCR: Real-time PCR; WT: Wild type.

Article Snippet: The levels of fibronectin 1 (FN1), Col I, and fibroblast activation protein alpha (FAP) in PSCs cell supernatant were detected by using Human FN ELISA Kit (No. E-EL-H0179c, Elabscience, Wuhan, China), Human Col I ELISA Kit (No. JM-03329H1, JINGMEI, Jiangsu, China), and Human FAPα ELISA Kit (No. EH3037, FineTest, Wuhan, China) according to the manufacturer's instructions.

Techniques: Migration, Software, Transwell Migration Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Activation Assay, Real-time Polymerase Chain Reaction

Journal: Chinese Medical Journal

Article Title: Exosomal miR-485-3p derived from pancreatic ductal epithelial cells inhibits pancreatic cancer metastasis through targeting PAK1

doi: 10.1097/CM9.0000000000002154

Figure Lengend Snippet: MiR-485-3p regulated PC cell migration and Invasion. (A, B) miR-485-3p was overexpressed and silenced In BxPC-3 and PANC-1 cells. (C, D) Transwell migration and Invasion assays in BxPC-3 and PANC-1 cells with overexpression or silencing of miR-485-3p (bar: 100 μm). The migrated or invaded cells were imaged at 200 × magnification in five random fields and counted using ImageJ software. (E) MiR-485-3p was overexpressed and silenced in PSCs. (F) FN1, COLIA1, and FAP levels in cell supernatant of PSCs transfected with miR-485-3p mimic or Inh-miR-485-3p were assessed by ELISA. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells.

Article Snippet: The levels of fibronectin 1 (FN1), Col I, and fibroblast activation protein alpha (FAP) in PSCs cell supernatant were detected by using Human FN ELISA Kit (No. E-EL-H0179c, Elabscience, Wuhan, China), Human Col I ELISA Kit (No. JM-03329H1, JINGMEI, Jiangsu, China), and Human FAPα ELISA Kit (No. EH3037, FineTest, Wuhan, China) according to the manufacturer's instructions.

Techniques: Migration, Over Expression, Software, Transfection, Enzyme-linked Immunosorbent Assay, Activation Assay